BernheimV1, Main, Exploration, bibRecord, 000033

Investigation et caracterisation de différents homologues de transporteurs ADP/ATP

Identifieur interne : 000033 ( Main/Exploration ); précédent : 000032; suivant : 000034

Investigation et caracterisation de différents homologues de transporteurs ADP/ATP

Auteurs : Aleksandra Woz Nicka-Misa Ila [France]

Source :

RBID : Hal:tel-01496246

Descripteurs français

mix :
- Cristallographie, Mitochondrie, Protéine membranaire, Structure 3D, Transport d'ADP/ATP.

English descriptors

mix :
- 3D structure, ADP/ATP transport, Crystallography, Membrane protein, Mitochondria.

Abstract

The main objective of this PhD project was to gain new structural data on the mitochondrial ADP/ATP carriers and develop tools for micro- and nano-crystallography approaches applied to membrane protein structural biology.The main role of the ADP/ATP carrier (AAC) is to import and export ADP3- and ATP4- respectively between the intermembrane space and the matrix through the inner mitochondrial membrane. AAC is the best characterized among all mitochondrial carriers. Much has been done to investigate its function and structure. However, since structural data are only available for one conformation of the protein some fundamental questions about the different conformational states adopted during the transport process still need to be answered.In this thesis we considered 4 human AAC homologs as a main target. They are involved in different genetic diseases but play also a role in cancerogenesis. This thesis describes and compares in detail the functional and structural characterization of the human AAC isoforms. It was an essential step to give insight into their native properties and is a precious starting point for the drug development field.Since the structural biology field is rapidly developing especially in serial crystallography techniques, there are more and more new applications for samples preparation, mounting and measurements in order to improve the quality of the data collected at the synchrotrons. Hence, our second objective was to use different membrane protein samples to develop new crystal-friendly crystallization set up combined with different sample environment on the beamline toward faster, more efficient and simpler data collection.

Url:

https://tel.archives-ouvertes.fr/tel-01496246

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Hal, to step Corpus: 000088
to stream Hal, to step Curation: 000088
to stream Hal, to step Checkpoint: 000032
to stream Main, to step Merge: 000033
to stream Main, to step Curation: 000033

Le document en format XML

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<front><div type="abstract" xml:lang="en">The main objective of this PhD project was to gain new structural data on the mitochondrial ADP/ATP carriers and develop tools for micro- and nano-crystallography approaches applied to membrane protein structural biology.The main role of the ADP/ATP carrier (AAC) is to import and export ADP3- and ATP4- respectively between the intermembrane space and the matrix through the inner mitochondrial membrane. AAC is the best characterized among all mitochondrial carriers. Much has been done to investigate its function and structure. However, since structural data are only available for one conformation of the protein some fundamental questions about the different conformational states adopted during the transport process still need to be answered.In this thesis we considered 4 human AAC homologs as a main target. They are involved in different genetic diseases but play also a role in cancerogenesis. This thesis describes and compares in detail the functional and structural characterization of the human AAC isoforms. It was an essential step to give insight into their native properties and is a precious starting point for the drug development field.Since the structural biology field is rapidly developing especially in serial crystallography techniques, there are more and more new applications for samples preparation, mounting and measurements in order to improve the quality of the data collected at the synchrotrons. Hence, our second objective was to use different membrane protein samples to develop new crystal-friendly crystallization set up combined with different sample environment on the beamline toward faster, more efficient and simpler data collection.</div>
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Serveur d'exploration Hippolyte Bernheim

Investigation et caracterisation de différents homologues de transporteurs ADP/ATP

Investigation et caracterisation de différents homologues de transporteurs ADP/ATP

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri